Project Description
Supervisors
Dr Marcela Hernandez Garcia, School of Biological Sciences, University of East Anglia
Dr Falk Hildebrand, Quadram Institute Bioscience
Dr Marc Dumont, University of Southampton
Scientific Background
Global warming and the potential end of the antibiotic era are two critical societal challenges. These two threats overlap because antibiotic contamination of the wider environment, in combination with climate warming, glacier retreat, and permafrost thaw, can open new niches for antibiotic resistant microbes to proliferate. We have found that microbial interactions shape the temporal dynamics of antimicrobial resistance (AMR) in the Arctic. Moreover, there is emerging evidence from terrestrial ecosystems that antibiotics and warming independently reduce the efficiency of microbial carbon use, which can translate to high C-efflux and lower potential for C-sequestration. In other words, both warming and antibiotic contamination may lead to the amplification of climate change. We predict that an increase in AMR in the Arctic will reduce microbial carbon use efficiency (CUE) because competitive interactions among microbes would lead to the allocation of energy and nutrients towards AMR instead of biomass growth. This PhD project will unravel the mechanisms driving AMR in pioneer and extreme soil environments in the Arctic, focussing on Svalbard – the fastest warming place on the planet, offering natural gradients in soil development, and pristine and anthropogenically influenced environments.
Research Methodology
The overarching aim is to understand whether climate warming and AMR impact microbial CUE. You will measure CUE by AMR bacteria in isolation and within Arctic soils. Additionally, you will investigate the potential for AMR bacteria in wastewater from Svalbard settlements to colonise Arctic soils and alter their CUE.
You will join sampling campaigns in Svalbard followed by training in cutting-edge tools for isolation and identification of microbes, including metagenomics and bioinformatics.
Training
Training will include cultivation of soil bacteria, quantitative PCR, amplicon-based sequencing, whole genome sequencing, and metagenomic analysis. You will present results at national and international conferences, and collaborate internationally with project partners abroad (including collaborators in France and Italy).
Person Specification
We are looking for a pro-active, highly motivated student. The successful candidate should have a strong background in microbial, soil or environmental sciences (e.g., BSc/Masters in Microbiology, Environmental Sciences, or similar). For fieldwork participation (non-essential), willing to join sampling campaigns in Svalbard.
Acceptable first degree subjects: BSc/Masters in Microbiology, Environmental Sciences, or similar
Project code: HERNANDEZ-GARCIA_UEA_ARIES26