Project Description
Supervisors
Dr Keiron Fraser, School of Biological and Marine Sciences, University of Plymouth
Dr Benjamin Ciotti, School of Biological and Marine Sciences, University of Plymouth
Professor Simon Lemarre, Faculty of Science, Université de Moncton, Canada
Scientific Background
Pollution of coastal waters by sewage is of major concern, but little is known about the biological impact on animals, or interactions with other stressors such as ocean warming. Growth is essential in all animals, allowing individuals to reach a threshold size for reproduction and occupy adult ecological niches. Soft-tissue growth is essentially achieved by the synthesis and retention of proteins, an energetically expensive process, typically accounting for 25-40% of the energy required by an animal. This study will make the first detailed analysis of how sewage pollution and warming seas are likely to affect protein metabolism and growth during the critical juvenile life stage, of the socioeconomically important, European seabass.
Research Methodology
Fish will be exposed to four water temperatures and to sewage pollution levels regularly detected in UK coastal waters that fail safe bathing standards (Escherichia coli >500 cfu/100ml; Intestinal enterococci >185 cfu/100ml). Experimental water temperatures will range from current UK summer temperatures, to those likely to occur in the UK in the next decades. Fish will be individually maintained in recirculating seawater aquaria and fed daily. At the end of the experiments, growth, protein synthesis and protein degradation will be measured.
In parallel, field-based measurements will be made of protein degradation pathways, and growth/protein synthesis proxies in wild fish at times of high and low sea temperature, in concert with measurements of seawater sewage contamination, to better understand how the detailed lab studies relate to fish living in the wild.
Training
This is an exceptional opportunity to develop highly relevant skills broadly spanning field and laboratory approaches to understand how human impacts affect animal populations. Training will be provided in field-based fish sampling techniques, fish husbandry , cutting-edge methods to measure protein synthesis utilising a deuterium-labelled phenylalanine flooding dose in Plymouth, as well as a range of advanced methods to measure protein degradation pathways, during a training visit to Canada.
Person Specification
We seek an enthusiastic individual with a degree in marine biology, biology, biochemistry or a related subject, a willingness to travel to field sites and preferably a UK valid driving licence.
Acceptable first degree subjects: Marine biology, biology, biochemistry, or a related subject.
Project code: FRASER_PLYM_ARIES26