Project Description
Supervisors
Dr Keiron Fraser, Biological and Marine Sciences (SoBMS), University of Plymouth – contact me
Dr Benjamin Ciotti, University of Plymouth
Professor Simon Lamarre, Université de Moncton Faculty of Science 18 Antonine-Maillet Ave, Moncton, NB E1A 3E9, Canada
Scientific background
Pollution of coastal waters by sewage is currently of major concern. However, little is known about the biological impact on critical functions in animals such as growth, particularly in combination with other stressors such as human-induced ocean warming. Growth is essential in all animals, allowing animals to reach a threshold size for reproduction and occupy adult ecological niches. Soft tissue growth in animals is essentially achieved by the synthesis and retention of proteins, an energetically expensive process, typically accounting for between 25 and 40% of the energy required by an animal. This study will make the first detailed analysis of how sewage pollution and warming seas are likely to affect protein metabolism and growth during the critical juvenile stage of development, in a species of substantial socioeconomic importance in the UK, European seabass.
Research methodology
The project will expose fish to four water temperatures and to sewage pollution levels that are regularly detected in UK coastal waters that fail safe bathing standards (Escherichia coli >500 cfu/100ml; Intestinal enterococci >185 cfu/100ml). Experimental water temperatures will range from current UK summer temperatures to temperatures that seabass are likely to experience in the UK in the next few decades. Fish will be individually maintained in recirculating seawater aquarium and fed daily. At the end of the experiments, growth, protein synthesis and protein degradation will be measured.
In parallel, field-based measurements will be made of protein degradation pathways in wild fish at times of high and low sea temperature, in concert with measurements of seawater sewage contamination, to better understand how the detailed lab studies relate to fish living in the wild.
Training
You will receive broad training in field-based fish sampling techniques, fish husbandry and a range of cutting-edge methods to measure protein synthesis utilising a deuterium-labelled phenylalanine flooding dose in Plymouth, as well as a range of advanced methods to measure protein degradation pathways, during a training visit to Canada.
Person specification
We seek an enthusiastic individual with a degree in marine biology, biology, biochemistry or a related subject and preferably a UK valid driving licence.
Acceptable first degree subjects: marine biology, biology, biochemistry, or a related subject.